Real-time monitoring of morphological plasticity
Although bacteria have evolved complex molecular strategies to maintain their shape, many are able to alter their shape and size as a survival strategy in response when they encounter stressful environments such as oxidative stress, nutrient limitation, DNA damage and antibiotic exposure.
With oCelloScope you can now monitor and analyse the bacterial growth and morphological changes simultaneously in standard 96 well plates. Through highly sensitive and automated data acquisition and image analysis, oCelloScope enables scientists to understand the effects and stress reactions of bacteria towards new antibiotic candidates in drug development, as well as on the existing antibiotic tested on putative resistant strains, without additional work load.
Below is an example of a full plate AST analyses performed with the BCA algorithm. Morphological changes can quickly be identified using the plate overview provided in our UniExplorer software. In well C5, a bump in the curve can be seen which corresponds to the spheroplast formation and cell burst seen in the video.