We develop with you
BioSense Solutions have created the oCelloScope as an open and flexible platform for use in R&D. The platform features a large lid for easy access and you can use most of your preferred micro-well plate formats.
We work with many science teams around the world and rely on input from our user community. Based on your input we develop new algorithms and tools to benefit all scientists in microbiology.
On this page you see the latest developments from BioSense Solutions and they are all products of joint user projects. Contact us if you have a specific need where automated imaging might present a solution.
oCelloScope Flow-Cell Starter Kit
The flow-cell technology was developed in a joint project discovering a new and innovative “At Line” technology for crystallization monitoring in a production environment. Since 2020 the technology has been adopted by scientists working with Biofilm, Urinary Tract Infections (UTI), Fermentation, Cell counting and not least as a basic tool to image samples in tough matrices (Molasses, Paint, Blood, Foods etc).
The flow-cells can be used with a micro-volume pump for non-static experiments or with end-stoppers. To accommodate new users we have made a Flow-Cell Starter Kit (Cat# USC.69.0009). The kit consists of an adapter for the instrument, 10×100 µm flow-cells, 10×800 µm flow-cells, end stoppers, tubing and a stand for easy pipetting.
Combining the flow-cells with a microvolume pump enable you to study microbials under non-static conditions. Above you see a biofilm build up under a constant flow. In similar experiments we also use valves to switch between medium and medium/drug.
The flow-cells accommodate a perfect distribution of cells. Cells or particles can be counted to calculate objects/ml.
Large Area Scanning
We have received several inquiries on scanning larger areas such as full wells. Our software developers have solved the technical challenge and we are now able to scan a variety of plates and slides. To give you an idea of magnitude, 1 full well image in a 24 well plate is stitched from 4000 images! In the image to the right you can see 3 different consumables for large area scanning. As always you can use your own flat bottomed micro-titer plates, which you already have in your lab. However, as we work with a tilted camera and light source technology you will see a shadow on one side. To accommodate this, we are developing and testing plates with conical wells. Plates and other consumables can be used with agar or in solution. The large area scanning can be used to give you an overview of your sample or to conduct dominance of species experiments. Some also monitor bacterial colony formation or interactions.
Full Well Scan of well in a 24 well plate. Image shows 2µl droplets of Lactobacillus and E-coli grown on agar. Zoom is made on interaction zone.
2µl E-coli droplet grown on agar in a 24 well plate. Time-lapse show colony development
Full Well Scan of well in a 24 well plate. Image shows yeast cells. Point here is to see the uneven distribution of cells in a 24 well micro-well plate format. Cells tend to crowd the edges.
Arabidopsis seedlings can be imaged over time to study growth or microbial/root interactions.
UniExplorer Tracking Tool
For the microbial R&D community we have created a tracking tool to study individual objects or groups of objects in a population over time. We use the X, Y positioning of objects in images to track and analyze development. Each object is given a track ID and we can use settings to sort for specific sizes or shapes. This unique tool is already in use with various fungal research teams to proportionate fungal spore populations upon fungicidal treatment.
On the left is an image of a fungal spore tracking. Left side shows the sample image from a given timepoint with selected spores in red. To the right in image you see the individual spores with images from every timepoint. Data can be exported and proportion of Dormant vs Swelling Vs Germinating spores can be quantified.
Below is an AST analysis combined with tracking of Pseudomonas aeruginosa (PA). On the left you see a growth kinetic experiment using the oCelloScope.PA has been treated with Imipenem at concentrations from 0,12mg/l to 64mg/l in addition to a positive and negative control. MIC values can be established in as little as 4 hours. In experiment we see the morphological responses and at high antibiotics concentration we see what might be persister cells. These we can follow throughout the experiment and use the tracking tool to monitor if they persist!