Skip to content
BioSense-Logo-Retina-2
Main Menu
  • Applications

      Microbiology

      • AST
      • Food Microbiology
      • Crop science
      • Fungal Material Science
      • General Microbiology
      • Automated fungal spore count
      • Automated bacteria count
      • AST
      • Food Microbiology
      • Crop science
      • Fungal Material Science
      • General Microbiology
      • Automated fungal spore count
      • Automated bacteria count

      Pharmaceuticals

      • API Precipitation
      • API Dissolution
      • Particle Size Distribution
      • API Precipitation
      • API Dissolution
      • Particle Size Distribution

      Production

      • Process optimization
      • Pharmaceutical bioburden
      • Process optimization
      • Pharmaceutical bioburden
  • Products

      oCelloScope™

      Fritlæg_OCS-removebg-preview

      µCount3D

      Accessories

      Mini_Incubator-removebg-preview
  • Publications
  • Company
      • About us
      • Distributors
      • Jobs
      • Contact
      • About us
      • Distributors
      • Jobs
      • Contact
  • Shop
  • Request a Quote
0,00 € 0 Cart

Crop science

Sustainable agriculture and pest control

Insects are a pest in agriculture and cause significant losses in crop yields and storage. A global need for new and efficient alternatives to chemical pesticides is driving the discovery of new bio pesticides. The oCelloScope is used by many agriculture companies in different BioAg applications.

Visualization of spore count of biopesticides with oCelloScope

SporeCount

SporeCount

Bio control agents such as Metharizium, Trichoderma, Coniothyrium, Isaria and Purpureocillium can be counted and quantified using the SporeCount function developed by BioSense Solutions. The SporeCount module is based on machine learning and is developed to count small round spores, normal sized spores or elongated spores. The algorithm can be used for final product enumeration or viability studies. SporeCount is able to count spores in many “dirty” samples or samples with hyphae.  Counts can be performed in solution in microtiter plates or on agar. Mini agar disks (Cat No. USC.69.0013) have been developed by BioSense solutions to image in 12-well plates and significantly save on consumables.

Easy and fast agricultural counts

Using a 96-well plate format you can use the counting algorithms to locate and count spores in wells. Contents can be visualized in software as actual number or concentration (objects/ml) – see red square to the right. A prerequisite for correct number is an even distribution of cells in wells. Distribution can be affected by several parameters such as pipetting and temperature differences of medium and plastic. When counting, it is recommended to use a large scan area (example 80 images = 3,6 mm) for better statistics.

Quantify germination in few easy steps

Prepare and pipette your samples into microtiter wells. Set your desired time intervals in the UniExplorer software and choose which algorithms to use. The results can be displayed in the software and exported to excel. 

A unique feature is to view results as percent. To the right 8 wells are analyzed. Algorithm count non-germinated spores and precise time of germination and proportion germinated in percent can easily be established. 

Engineered microbes and improved yields

The Rhizomicrobiome is contributing to yield and productivity in different ways. Microorganisms can improve soil fertility, texture, nutrient acquisition, and secretion of intra and extracellular molecules and metabolites. The oCelloScope is used to optimize growth of engineered strains, determine morphology adaptations and to look at interactions using a non-invasive technology. 

To the right you see a sample from an infested garlic. There are several fungal species in sample. In focus you see Fusarium, a plant pathogen responsible for significant yield losses in crops.    

Visualization of individual cell tracking with oCelloScope software

Tracking of individual cells

In BioAG R&D knowing how spores develop from dormant to isotropic growth has historically been a “black box”. With the new tracking module in UniExplorer you can now track each spore in a population over time. You get to see and quantify development using an array of different parameters. In addition, we present your data in histograms for particle size distribution. 

Tip! When making deeper analysis we recommend to load an existing job to run the analysis on. Being able to see the images when you program your settings greatly improves results. Remember that you are always free to re-analyze any job with any algorithm.    

Testing new antifungals in BioAg

Teams around the world are developing new and green biopesticides to replace the extensive use of chemicals. With the oCelloScope you can test both species and new  substances on pathogenic species. You get analysis and images to support your research. On the right you see a fungal pathogen treated with different concentrations of compound X. Initial increase in curve is settling of spores on the bottom of plates. Dropping of curve means germination of spores and experiment shows effectiveness of compound at different concentrations. 

Tip! If you need a special algorithm for species detection please reach out to our custom development team. 

Bacillus viability and growth kinetics

In BioAg, multiple commercial products contain Bacillus species. On the left we test the viability of a commercial product containing Bacillus subtilis and Bacillus paralicheniformis. Product is supplied as spore suspension. Experiment is carried out in 96-well plate and spores are initially seen as small dots. After a few hours in liquid medium, spores enter vegetative growth and growth is measured. Spores can also be grown on agar using our mini agar disks and colony tracking module. Below is a zoom of three times points. Speed of growth varies between spores.

 

Machine Learning Mycelium Tracker

BioSense Solutions have developed a new deep learning module to accurately find mycelium in an image. Quantifications can be one well in time lapse or several wells compared. Output is given as area covered, volume (biomass), number of tips (indication of branching) and hyphal width. Module is currently used for fermentation optimization, change of morphology studies and fungal fingerprinting.  

Image show mycelium of Trichoderma Harzianum on a PDA surface with overlay from Mycelium Tracker. 


2D Co-culture

Co-culture experiment using the oCelloScope and 24 well plates. Spores of Trichoderma harzianum and Botrytis cinerea was first adjusted to 5.0E+04 using the µCount3D instrument. Subsequently spores were set on a 2D surface in wells. The oCelloScope was set to image every 20 minutes post germination. Video helps unravel how Trichoderma attacks Botrytis on a surface.

Fast CFU/ml Assay

BioAg fermentation often get contaminated by unwanted bacteria. With the IntuGrow assay you get to determine CFU/ml in hours instead of days. Use your selective agar with our mini agar disks and our dedicated software will count bacterial growth over time. Method has been validated against EU GMP method and is in routine use at pharmaceutical production sites.   

IntuGrow Assay

Ready to get started?

We are ready to help you solve your tough research challenges. Our highly qualified team can help advance your next big discovery.

Request a Quote
BioSense Solutions ApS

Hirsemarken 1
DK-3520 Farum
Denmark

  • Terms & Conditions
Contact us:
  • Phone: (+45) 26281943
  • E-mail: info@biosensesolutions.dk

Follow us on LinkedIn

Linkedin
  • CVR / VAT: DK38602926
  • My account
Manage Cookie Consent
We use technologies like cookies to store and/or access device information. We do this to improve browsing experience and to show (non-) personalized ads. Consenting to these technologies will allow us to process data such as browsing behavior or unique IDs on this site. Not consenting or withdrawing consent, may adversely affect certain features and functions.
Functional Always active
The technical storage or access is strictly necessary for the legitimate purpose of enabling the use of a specific service explicitly requested by the subscriber or user, or for the sole purpose of carrying out the transmission of a communication over an electronic communications network.
Preferences
The technical storage or access is necessary for the legitimate purpose of storing preferences that are not requested by the subscriber or user.
Statistics
The technical storage or access that is used exclusively for statistical purposes. The technical storage or access that is used exclusively for anonymous statistical purposes. Without a subpoena, voluntary compliance on the part of your Internet Service Provider, or additional records from a third party, information stored or retrieved for this purpose alone cannot usually be used to identify you.
Marketing
The technical storage or access is required to create user profiles to send advertising, or to track the user on a website or across several websites for similar marketing purposes.
Manage options Manage services Manage {vendor_count} vendors Read more about these purposes
View preferences
{title} {title} {title}