Gå til indholdet
BioSense-Logo-Retina-2
Main Menu
  • Applications

      Microbiology

      • AST
      • Food Microbiology
      • Agriculture
      • Fungal Material Science
      • General Microbiology
      • Automated Spore Count
      • AST
      • Food Microbiology
      • Agriculture
      • Fungal Material Science
      • General Microbiology
      • Automated Spore Count

      Pharmaceuticals

      • API Precipitation
      • API Dissolution
      • Particle Size Distribution
      • API Precipitation
      • API Dissolution
      • Particle Size Distribution

      Production

      • Process optimization
      • Pharmaceutical bioburden
      • Process optimization
      • Pharmaceutical bioburden
  • Products

      oCelloScope™

      oCelloScope open

      LattiScope™

      image003

      Accessories

      4slideholder
  • Publications
  • Company
      • About us
      • Distributors
      • News
      • Contact
      • About us
      • Distributors
      • News
      • Contact
  • Request a Quote

Automated Spore Count

Banner Image: Courtesy of Westerdijk Fungal Biodiversity Inst


Counting spores in fungal samples

Fungal cell counts are used in both R&D and production settings in a wide variety of applications. Most cell counts are based on manual microscopy and this we aim to change. BioSense Solutions employ machine learning algorithms to count spores. Spores can even be counted in dirty samples containing hyphae, fibres or other debris. If work is performed in micro titer plates, and we know the sample volume, spores/ml can be automatically calculated. Counts can be performed as end-point or used in time-lapse studies to quantify germination.   

The oCelloScope is routinely used to count final products in both the brewing industry and in global agriculture (Bio pest control). 

Even distribution of cells is paramount in getting correct results. It is well-established that cells can populate the edges of a well and thereby affect count. Literature finds that edge effects are minimized if sample and plate have same temperature. In addition, pipetting into wells can affect distribution.     

End-point SporeCount

With an even distribution of cells, triplicates will yield similar results. In the software, wells are shown and findings can be displayed as actual number or spores/ml. Spores/ml is calculated from sample volume. 

Note that when you inoculate 100µl sample into a well of a 96-well plate, spores will settle at different rates. To get precision in your count it is advised to wait 1h before the SporeCount analysis is run. It is advised to increase number of images in wells from 10 to 70 to capture a larger area in wells. In addition, it is given that plates are quality manufactured and have an even surface.

Triplicate counts of spores in a 96-well plate is visualized on the left – both as actual number and spores/ml. 

Time-Lapse SporeCount

A common application using the SporeCount module is to time-lapse your spore development. Spores will be tracked until the time-point where they develop a germ-tube. What you get is exact time of germination and you are able to easily quantify germination. Resulting curve on the left can be set to show actual spore count or percentage. When set to percentage you can quantify how many spores in a population will germinate.  

Note how curve increase from starting point. This is due to settling of spores in wells. This can take hours depending on fungal species.  

From Dormant to Isotropic growth

The SporeCount module is based on Deep Learning algorithms. Algorithms are designed to track spores from dormant to the point where they develop a germ-tube. Algorithms are species specific for highest level of precision. As fungal spores comes in a variety of shapes, we offer to custom build deep learning algorithms for your species – contact us to learn more. 

 

Ready to get started?

We are ready to help you solve your tough research challenges. Our highly qualified team can help advance your next big discovery.

Request a Quote
BioSense Solutions ApS

Hirsemarken 1
DK-3520 Farum
Denmark

Contact us:
  • Phone: (+45) 26281943
  • E-mail: info@biosensesolutions.dk

Follow us on LinkedIn

Linkedin
  • CVR / VAT: DK38602926
Administrer samtykke til cookies
Vi bruger cookies til at optimere vores websted og vores service.
Funktionsdygtig Altid aktiv
Den tekniske lagring eller adgang er strengt nødvendig med det legitime formål at muliggøre brugen af en specifik tjeneste, som abonnenten eller brugeren udtrykkeligt har anmodet om, eller udelukkende med det formål at overføre en kommunikation via et elektronisk kommunikationsnet.
Præferencer
Den tekniske lagring eller adgang er nødvendig for det legitime formål at lagre præferencer, som abonnenten eller brugeren ikke har anmodet om.
Statistikker
Den tekniske lagring eller adgang, der udelukkende anvendes til statistiske formål. Den tekniske lagring eller adgang, der udelukkende anvendes til anonyme statistiske formål. Uden en stævning, frivillig overholdelse fra din internetudbyders side eller yderligere optegnelser fra en tredjepart kan oplysninger, der er gemt eller hentet til dette formål alene, normalt ikke bruges til at identificere dig.
Marketing
Den tekniske lagring eller adgang er nødvendig for at oprette brugerprofiler med henblik på at sende reklamer eller for at spore brugeren på et websted eller på tværs af flere websteder med henblik på lignende markedsføringsformål.
Vælg muligheder Manage services Vælg forhandler Læs mere om disse formål
Præferencer
{title} {title} {title}